| 应用微阵列芯片检测与2型糖尿病相关的单核苷酸多态性 |
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| 引用本文： | 陈琰,刘桂锋,刘霞,张桂珍,王振新.应用微阵列芯片检测与2型糖尿病相关的单核苷酸多态性[J].高等学校化学学报,2013,34(5):1078-1083. |
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| 作者姓名： | 陈琰 刘桂锋 刘霞 张桂珍 王振新 |
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| 作者单位： | 1. 吉林大学中日联谊医院中心研究室, 长春 130033;
2. 吉林大学中日联谊医院放射线科, 长春 130033;
3. 中国科学院长春应用化学研究所, 电分析国家重点实验室, 长春 130022;
4. 吉林大学第二医院内分泌科, 长春 130042 |
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| 基金项目： | 国家自然科学基金(批准号: 21075118)资助. |
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| 摘 要： | 基于三维(3D)寡核苷酸微阵列芯片的荧光检测法, 研制了一种用于筛选能检测2型糖尿病的特定寡核苷酸探针. 使用第4代(G4)聚(酰胺-胺)(PAMAM)树枝状大分子修饰的载玻片为基底, 以氨基修饰的寡核苷酸为固定探针构建3D寡核苷酸微阵列芯片. 采用荧光化合物Cy5修饰的寡核苷酸为检测探针获得荧光信号. 以2型糖尿病易感基因TCF7L2的rs7903146位点为研究对象, 通过对含有16种(8对)寡核苷酸的寡核苷酸文库的筛选, 获得了1对能用于2型糖尿病检测的寡核苷酸探针. 通过单核苷酸多态性和等位基因分析证明, 该寡核苷酸探针对靶标寡核苷酸检测具有高特异性, 并能准确检测低至2%的等位基因频率.
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| 关 键 词： | 寡核苷酸微阵列 2型糖尿病 等位基因频率 核苷酸探针 |
| 收稿时间： | 2013-01-08 |
Detecting Type 2 Diabetes-related Single Nucleotide Polymorphisms by Microarray |
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| CHEN Yan,LIU Gui-Feng,LIU Xia,ZHANG Gui-Zhen,WANG Zhen-Xin.Detecting Type 2 Diabetes-related Single Nucleotide Polymorphisms by Microarray[J].Chemical Research In Chinese Universities,2013,34(5):1078-1083. |
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| Authors: | CHEN Yan LIU Gui-Feng LIU Xia ZHANG Gui-Zhen WANG Zhen-Xin |
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| Institution: | 1. Central Research Department, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
2. Radiology Department, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
3. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China;
4. Endocrinology Department, The Second Hospital of Jilin University, Changchun 130042, China |
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| Abstract: | A three-dimensional(3D) oligonucleotide microarray-based fluorescent assay was developed for screening specific oligonucleotide probes for type 2 diabetes detection. The 3D oligonucleotide microarray was fabricated on the home-made fourth-generation(G4) NH2-terminated poly(amidoamine) dendrimers(PAMAM) modified substrate. Assay for rs7903146 site of the type 2 diabetes related gene TCF7L2 was developed. The utility of this assay was demonstrated with an oligonucleotide library, a collection of 16(8 pairs) oligonucleotides, and one specific oligonucleotide probe pair for type 2 diabetes was obtained. Taking advantage of the high specificity of the probe pair, the allele frequency as low as 2% can be accurately determined. |
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| Keywords: | Oligonucleotide microarray Type 2 diabetes Allele frequency Oligonucleotide probe |
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