| 稀土离子对重组内皮抑素与肝素相互作用的影响 |
| |
| 引用本文： | 王群,倪嘉缵.稀土离子对重组内皮抑素与肝素相互作用的影响[J].高等学校化学学报,2004,25(10):1783-1788. |
| |
| 作者姓名： | 王群 倪嘉缵 |
| |
| 作者单位： | 1. 中国科学院长春应用化学研究所, 稀土化学与物理国家开放实验室, 长春130022;
2. 深圳大学生命科学学院, 深圳518060 |
| |
| 基金项目： | 国家自然科学基金重大项目 (批准号 :2 9890 2 80 )资助 |
| |
| 摘 要： | 通过荧光光谱法和圆二色谱法研究了重组内皮抑素与稀土离子和肝素的作用.结果表明,稀土离子和肝素都可以与重组内皮抑素结合并引起蛋白的荧光猝灭和二级结构的改变,稀土离子与重组内皮抑素的结合会影响其与肝素的相互作用,并降低重组内皮抑素对人脐静脉内皮细胞的增殖抑制活性.
|
| 关 键 词： | 重组内皮抑素 荧光光谱 圆二色谱 稀土离子 内皮细胞 |
| 文章编号： | 0251-0790(2004)10-1783-06 |
| 收稿时间： | 2004-05-19 |
Effect of Lanthanides on the Interaction Between Recombinant Endostatin and Heparin |
| |
| WANG Qun ,NI Jia-Zuan.Effect of Lanthanides on the Interaction Between Recombinant Endostatin and Heparin[J].Chemical Research In Chinese Universities,2004,25(10):1783-1788. |
| |
| Authors: | WANG Qun NI Jia-Zuan |
| |
| Institution: | 1. State Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. College of Life Science, Shenzhen University, Shenzhen 518060, China |
| |
| Abstract: | The interaction of recombinant endostatin with lanthanides and heparin was studied by fluorescence and CD spectra. The results indicate that the binding of lanthanides, heparin to recombinant endostatin(rhES) causes quenching of its fluorescence and conformational changes of rhES.The binding of lanthanides to rhES can affect the interaction between its protein and heparin and decrease the inhibiting activity of rhES to the proliferation of human umbilical vein endothelial cell. |
| |
| Keywords: | Recombinant endostatin Fluorescence spectra Circular dichroism spectra Lanthanides Endothelial cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《高等学校化学学报》浏览原始摘要信息 |
| 点击此处可从《高等学校化学学报》下载免费的PDF全文 |
