| 高效液相色谱-激光诱导荧光-增强型电荷耦合器件检测氨基酸的研究 |
| |
| 引用本文： | 熊少祥,韩慧婉,赵睿,王光辉,马会民,陈义,刘国诠.高效液相色谱-激光诱导荧光-增强型电荷耦合器件检测氨基酸的研究[J].高等学校化学学报,2000,21(8):1191-1195. |
| |
| 作者姓名： | 熊少祥 韩慧婉 赵睿 王光辉 马会民 陈义 刘国诠 |
| |
| 作者单位： | 中国科学院化学研究所分子科学中心, 北京 100080 |
| |
| 基金项目： | 国家自然科学基金! (批准号 :2 9675 0 3 0 )，中国科学院“九五”重大基础研究项目! (批准号 :KJ95 1-A1-5 0 7) |
| |
| 摘 要： | 以3-对羟基苯甲酰喹啉-2-甲醛(CBQCA)为检测痕量氨基酸的柱前衍生试剂,利用高效液相色谱-激光诱导荧光-增强型电荷耦合器件检测器(HPLC-LIF-ICCD)系统,考察了氨基酸衍生和HPLC分离过程中多种因素的影响,建立了HPLC-LIF-ICCD分离检测痕量氨基酸的新方法,并应用于鼠脑微透析液的氨基酸检测.当信噪比为3时,对甘氨酸的质量检出限为5fmol.
|
| 关 键 词： | 高效液相色谱 激光诱导荧光 增强型电荷耦合器件 分离 微透析 |
| 文章编号： | 0251-0790(2000)08-1191-05 |
| 收稿时间： | 1999-09-22 |
| 修稿时间： | 1999年9月22日 |
Studies on the Determination of Amino Acids by High Performance Liquid Chromatography with Laser Induced Fluorescence-Intensified Charge Coupled Device Detector |
| |
| XIONG Shao-Xiang,HAN Hui-Wan,ZHAO Rui,WANG Guang-Hui,MA Hui-min,CHEN Yi,LIU Guo-quan.Studies on the Determination of Amino Acids by High Performance Liquid Chromatography with Laser Induced Fluorescence-Intensified Charge Coupled Device Detector[J].Chemical Research In Chinese Universities,2000,21(8):1191-1195. |
| |
| Authors: | XIONG Shao-Xiang HAN Hui-Wan ZHAO Rui WANG Guang-Hui MA Hui-min CHEN Yi LIU Guo-quan |
| |
| Institution: | Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China |
| |
| Abstract: | A High performance liquid chromatography-laser induced fluorescence-intensified charge coupled device(HPLC-LIF-ICCD) system was used for the analysis of amino acid. Using 3-(4-carboxybenzoyl)-2-quinoline-carboxyaldehyde(CBQCA) as a derivatizing agent, several factors influencing the derivatization and separation of amino acid were investigated in detail. The mass detection limit of glycine was 5×10-15 mol. The method was applied to the detection of amino acids in rat brain microdialysates. |
| |
| Keywords: | High performance liquid chromatography Laser induced fluorescence Intensified charge coupled device Separation Microdialysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《高等学校化学学报》浏览原始摘要信息 |
| 点击此处可从《高等学校化学学报》下载免费的PDF全文 |
