| Abstract: | The synthesis of a novel tris(bidentate ligand)ruthenium(II) complex 7 and its efficient tethering to the 5′-end of Oligonucleotides is described. The resulting Δ- and Δ-isomeric ruthenium(II)-labelled Oligonucleotides 10a--c and 11a--d were separated either by reversed-phase HPLC or by polyacrylamide gel electrophoresis. The diastereoisomerically pure isomers were fully characterized by UV/VIS and CD spectroscopy, mass spectrometry, and enzymatic digestion with base analysis. We also investigated the thermal denaturation of the hybridized double strands. |
