Photochemically-induced crystallization of protein |
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| Institution: | 1. State Key Laboratory of Silicon Materials, Department of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China;2. Key Laboratory of Advanced Materials and Applications for Batteries of Zhejiang Province, China;3. School of Materials Science and Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100191, China;1. Dongguan Cleaner production Center, Dongguan University of Technology, Dongguan, Guangdong 523808, China;2. Beijing Research Institute of Chemical Industry, SINOPEC, Beijing 100013, China;1. Institute of Physics, University of Tartu, Ravila 14с, 50411 Tartu, Estonia;2. Physics Faculty, Moscow State University, Leninskiye Gory 1-2, 11991 Moscow, Russia;3. Skobeltsyn Institute of Nuclear Physics, Moscow State University, Leninskiye Gory 1-2, 11991 Moscow, Russia;4. A. Alikhanyan National Science Laboratory, Yerevan Physics Institute, Alikhanyan Yeghbayrneri St. 2, 0036 Yerevan, Armenia;5. College of Sciences, Chongqing University of Posts and Telecommunications, 400065 Chongqing, PR China;6. Institute of Physics, Jan Dlugosz University, Armii Krajowej 13/15, PL-42200 Czestochowa, Poland;7. Institute of Physics, Polish Academy of Sciences, Al. Lotników 32/46, 02-668 Warsaw, Poland;8. Central Research and Development Institute of Chemistry and Mechanics, Nagatinskaya St. 16a, 115487 Moscow, Russia;9. Institute of Light and Matter, CNRS, University Lyon1, 69622 Villeurbanne, France;1. State Key Laboratory for Silicon Materials and Center for Electron Microscopy, Department of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, China;2. Hangzhou Bluestar New Materials Technology Co., Ltd, Hangzhou 310012, China;3. Weihai Blue Star Glass Co., Ltd, Weihai 264205, China;1. Laboratoire de Physique Statistique de l''École Normale Supérieure, UPMC, Université Paris Diderot, CNRS, UMR 8550, Paris, France;2. Laboratoire de Cristallographie et RMN Biologiques, Université Paris Descartes, CNRS, UMR 8015, Paris, France;3. Institut Charles Sadron - UPR 022 – CNRS - Unistra, Strasbourg, France;4. Sorbonnes Université Univ Paris 6, UMR 7371, UMR_S 1146, Laboratoire d''Imagerie Biomédicale, Paris, France;5. CNRS, UMR 7371, Laboratoire d''Imagerie Biomédicale, Paris, France;6. INSERM, UMR_S 1146, Laboratoire d''Imagerie Biomédicale, Paris, France |
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| Abstract: | I demonstrate photochemically induced crystallization of metastable hen egg-white lysozyme solution by weak UV irradiation for several tens seconds. The most effective irradiation time range is 10–60 s, and in this range the enzyme activity is maintained. Intermediates, neutral radicals at tryptophan residual produced by one-photon absorption, enhance nucleation. When the intermediate is selectively excited by visible light, the intermediate is denatured. At that time the light-induced nucleation is inhibited. This result indicates the intermediate induces nucleation. The radical forms lysozyme dimer that is detected by an SDS-PAGE electrophoresis experiment. An addition of polyethylene glycol (PEG) greatly enhances light-induced nucleation. PEG affects to shorten the intermediate radical lifetime, which suggests that PEG assists to form dimer. We consider that the photochemical dimer behaves as smallest cluster to grow critical nucleus. The smallest cluster formation is the rate determining step in classical nucleation theory due to surface energy disadvantage. The photochemical dimer is formed by a covalent bond, and the nucleation is initiated from stable dimer. The nucleation enhancement is reasonably explained. The present researches results point out the development of a new method for controlling nucleation and growth that could be applied for structural genomics and pharmaceutical industry for instance. |
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