PICOSECOND LASER PHOTOLYSIS STUDIES OF FLUORESCENCE QUENCHING MECHANISMS OF FLAVIN: A DIRECT OBSERVATION OF INDOLE-FLAVIN SINGLET CHARGE TRANSFER STATE FORMATION IN SOLUTIONS and FLAVOENZYMES |
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| Authors: | Akiya Karen Noriaki Ikeda Noboru Mataga Fumio Tanaka† |
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| Institution: | Department of Chemistry, Faculty of Engineering Science, Osaka University, Toyonaka, Osaka 560;Mie Nursing College, Tsu, Mie 514, Japan |
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| Abstract: | Formation of singlet charge transfer (heteroexcimer) states in the course of the fluorescence quenching of lumiflavin and riboflavin tetrabutyrate by indole and N-methylindole have been directly observed by means of time-resolved absorption spectral measurements using a picosecond laser photolysis method. Similar transient spectra have been observed also in the case of a flavoenzyme, D-amino acid oxidase. |
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