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Photoinactivation of Moraxella catarrhalis Using 405-nm Blue Light: Implications for the Treatment of Otitis Media |
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| Authors: | Xiaojing Liu Qihang Chang Raquel Ferrer-Espada Leon G Leanse Xueping Sharon Goh Xiuli Wang Jeffrey A Gelfand Tianhong Dai |
| Institution: | 1. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA;2. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA
Tongji University School of Medicine, Shanghai, China;3. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA Vaccine and Immunotherapy Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA;4. Institute of Photomedicine, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, China;5. Vaccine and Immunotherapy Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA |
| Abstract: | Moraxella catarrhalis is one of the major otopathogens of otitis media (OM) in childhood. M. catarrhalis tends to form biofilm, which contributes to the chronicity and recurrence of infections, as well as resistance to antibiotic treatment. In this study, we aimed to investigate the effectiveness of antimicrobial blue light (aBL; 405 nm), an innovative nonpharmacological approach, for the inactivation of M. catarrhalis OM. M. catarrhalis either in planktonic suspensions or 24-h old biofilms were exposed to aBL at the irradiance of 60 mW cm−2. Under an aBL exposure of 216 J cm−2, a >4-log10 colony-forming units (CFU) reduction in planktonic suspensions and a >3-log10 CFU reduction in biofilms were observed. Both transmission electron microscopy and scanning electron microscopy revealed aBL-induced morphological damage in M. catarrhalis. Ultraperformance liquid chromatography results indicated that protoporphyrin IX and coproporphyrin were the two most abundant species of endogenous photosensitizing porphyrins. No statistically significant reduction in the viability of HaCaT cells was observed after an aBL exposure of up to 216 J cm−2. Collectively, our results suggest that aBL is potentially an effective and safe alternative therapy for OM caused by M. catarrhalis. Further in vivo studies are warranted before this optical approach can be moved to the clinics. |
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