Synergistic Effect of MWNTs/CeO2/CHIT Film for Detection of CdSe Nanoparticle Labeled Sequence‐Specific of 35S Promoter of Cauliflower Mosaic Virus Gene |
| |
| Authors: | Tao Yang Daming Huang Qianhe Li Wei Zhang Kui Jiao |
| |
| Institution: | Key Laboratory of Eco‐chemical Engineering (Ministry of Education), College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.?R. China |
| |
| Abstract: | A porous composite film was fabricated combining the advantages of multiwalled carbon nanotubes, CeO2 and chitosan. The synergistic effect of the film improved the immobilization of probe ssDNA. The loaded probe ssDNA was used for detection of CdSe quantum dots labeled target DNA. The DNA hybridization reaction was detected by differential pulse anodic stripping voltammetry of Cd2+ after the oxidative release of labeled CdSe quantum dots. The established DNA biosensor can discriminate different target sequences associated with 35S promoter of cauliflower mosaic virus gene with relatively wide linear range and low detection limit (2.4×10?13 mol/L). |
| |
| Keywords: | Synergistic effect Cerium dioxide DNA biosensor CdSe quantum dots Differential pulse anodic stripping voltammetry |
|
