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Electrochemical Redundant Microsensor Arrays for Glucose Monitoring with Patterned Polymer Films |
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| Authors: | Amos Mugweru Becky?L Clark Michael?V Pishko |
| Institution: | 1. Department of Chemistry and Biochemistry, Rowan University, 201 Mullica Hill Road, Glassboro, NJ 08028, USA;2. Department of Chemical Engineering, Pennsylvania State University, 263 Materials Research Laboratory, University Park, PA 16802, USA;3. Department of Chemical Engineering, Chemistry, and Material Science and Engineering, Pennsylvania State University, 204 Fenske Laboratory, University Park, PA 16802, USA |
| Abstract: | Redundant microsensor arrays for glucose sensing were fabricated using photopolymerization of poly(ethylene glycol) diacrylate (PEG‐DA) with 2‐hydroxy‐2‐methyl phenyl‐propanone as photoinitiator to encapsulate the enzyme glucose oxidase. Silicon micro fabrication technologies were used to fabricate microelectrode sensor arrays on flexible polyimide sheets. These microarray sensors were individually addressable as observed using square‐wave voltammetry. Redox polymer, poly4‐vinylpyridine Os(bipyridine)2Cl]‐co‐ethylamine, was first immobilized on the electrode surface and then glucose oxidase was entrapped in PEG‐DA hydrogels. The redox polymer was found to exchange electrons with glucose oxidase in biocompatible PEG‐DA hydrogels. The entrapped glucose oxidase was found to respond linearly to glucose in solution (0–20 mM) as determined using square‐wave voltammetry. |
| Keywords: | Electrochemical biosensor Arrays of microsensors Glucose Photolithography Biocompatible hydrogel |