Determination of Anti-Parkinson Drug Pramipexole Using a Label-free Biosensor and Evaluation of its Interaction with ds-DNA |
| |
| Authors: | Hadi Mahmoudi-Moghaddam Zahra Garkani-Nejad |
| |
| Institution: | Chemistry Department, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, 7616913439 Iran |
| |
| Abstract: | In this study, we fabricated an effective and sensitive DNA biosensor based on flower-like Pt/NiCo2O4 modified carbon paste electrode (FL-Pt/NiCo2O4/CPE) for detection of pramipexole (PPX). Spectrophotometry, differential pulse voltammetry (DPV) and docking methods were employed to evaluate the interaction of DNA-PPX. Moreover, the DPV technique was chosen to monitor the electrochemical response of guanine on the DNA biosensor. The relationship between the concentration of PPX and the oxidation signal of guanine was linear in the range of 0.4 to 310.0 μM and a limit of detection (LOD) of 0.09 μM was calculated. |
| |
| Keywords: | Pramipexole Biosensor DNA Interaction Docking |
|
|
