Electrochemical detection of asparaginase antibodies using bifunctionalized carbon interfaces |
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Authors: | Daisy Marshall Joshua Lehr |
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Institution: | School of Science, Engineering and Environment, University of Salford, 43 Crescent, Salford, M5 4WT United Kingdom |
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Abstract: | The early detection of anti-asparaginase biomarker can facilitate timely modification of asparaginase chemotherapy, thereby avoiding serious complications. Herein we describe the preparation of a novel electrochemical biosensing interface for rapid detection of anti-asparaginase in the picomolar range (1–10 000 pM). Coimmobilization of ferrocene and asparaginase on a carbon interface (via diazonium grafting) facilitates transduction through attenuation of the surface-bound ferrocene redox couple. The limit of detection of 0.8 pM for this point-of-care applicable method compares favourably to that of traditional faradaic assaying (2.0 pM) where transduction occurs by the target blocking the diffusion of the solution redox probe Fe(CN)6]3−/4−. |
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Keywords: | anti-asparaginase asparaginase biosensor diazonium leukaemia |
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