Nylon Nanofibrous Biosensors for Glucose Determination |
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Authors: | Matteo Scampicchio Alessandra Arecchi Andrea Bianco Andrea Bulbarello Chiara Bertarelli Saverio Mannino |
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Institution: | 1. Università degli Studi di Milano, Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Via Celoria, 2, 20133?Milan, Italy;2. Politecnico di Milano, Dipartimento di Chimica Materiali ed Ingegneria Chimica Milan, Italy;3. DSM Nutritional Products Ltd., P.?O. Box 2676, Basel, Switzerland |
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Abstract: | Nanofibrous membranes have been produced by electrospinning to develop first generation glucose biosensors. The direct immobilization of glucose oxidase onto the polyamide nanofibrous surfaces by drop coating revealed a simple and efficient method for the development of sensitive, stable, and reproducible electrochemical biosensors. The biosensor showed a linear response over the range 1–9×10?3 glucose (R2=0.9997) with a sensitivity of 1.11 μA/mM and a limit of detection of 2.5×10?6 M (S/N=3). The uncertainty of repeatability was 2% (RSD%, n=30). After one month of storage, the signal decreased of 35%. The recovery of glucose, evaluated in real samples of honey, was 98% (RSD%=1%, n=3). |
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Keywords: | Electrospinning Nylon‐6 Glucose oxidase Nanofibrous membrane Amperometric detection Membranes Biosensors |
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