Ultrasensitive Eletrogenerated Chemiluminescence Immunoassay by Magnetic Nanobead Amplification |
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| Authors: | Mingyue Li Yuming Sun Li Chen Lu Li Guizheng Zou Xiaoli Zhang Wenrui Jin |
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| Institution: | 1. School of Chemistry and Chemical Engineering, , Shandong University, Jinan 250100, P.?R. China;2. Information Science and Engineering School, Shandong University, Jinan 250100, P.?R. China |
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| Abstract: | An ultrasensitive electrogenerated chemiluminescence (ECL) immunoassay was proposed by using magnetic nanobeads (MNBs) as the carrier of ECL labels for ECL emission amplification. Carcinoembryonic antigen (CEA) and MNBs were initially immobilized on a platform in 1 : 1 molar ratio via sandwich immunoreaction. Subsequently, the MNBs were released from the platform and labeled with Ru(bpy)32+ species. After the MNBs with Ru(bpy)32+ were immobilized on an Au electrode, ECL of the Ru(bpy)32+ was measured for CEA determination. A linear relation between the ECL intensity and CEA concentration was obtained in a range of 1×10?14 to 3×10?13 mol/L (2.0 to 60 pg/mL) with a limit of detection of 8.0×10?15 mol/L (1.6 pg/mL). |
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| Keywords: | Electrogenerated chemiluminescence Immunoassays Magnetic nanobead Carcinoembryonic antigen Luminescence |
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