Capillary electrophoresis as a tool for screening aptamer with high affinity and high specificity to ricin |
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| Authors: | Jijun Tang Jianwei Xie Lei Guo Yan Yan Ningsheng Shao |
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| Institution: | (1) Beijing Institute of Pharmacology & Toxicology, Beijing, 100850, China;(2) Beijing Institute of Basic Medical Sciences, Beijing, 100850, China |
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| Abstract: | Aptamers which specifically recognize targets are selected from random oligonucleotide library using systematic evolution
of ligands by exponential enrichment (SELEX). In this paper, capillary electrophoresis (CE) as a separation approach has been
introduced to SELEX procedure. The high efficiency of CE gives rise to greatly shorten the selection procedure. The results
from enzyme-linked assay and dot blot experiment show that an enrichment pool has been obtained after four rounds selection,
which can specifically recognize ricin.
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Translated from Chemical Journal of Chinese Universities, 2006, 27(10): 1,840–1,843 译自: 高等学校化学学报] |
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| Keywords: | aptamer systematic evolution of ligands by exponential enrichment (SELEX) capillary electrophoresis (CE) single-stranded DNA (ssDNA) pool ricin |
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