Site-directed cleavage of DNA by an oligonucleotide conjugate with o-bromobenzoic acid in the presence of copper ions |
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| Authors: | Koval O A Chernolovskaya E L Litvak V V Vlassov V V |
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| Institution: | (1) Novosibirsk Institute of Bioorganic Chemistry, Siberian Branch of the Russian Akademy of Sciences, 8 ul. Akad. Lavrentieva, 630090 Novosibirsk, Russian Federation |
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| Abstract: | Site-directed cleavage of single- and double-stranded DNAs by an oligonucleotide conjugate with 5-N-(3-aminopropyl)sulfamoyl]-2-bromobenzoic acid was investigated. When forming duplex complexes with a single-stranded DNA and triplex complexes with a double-stranded DNA, this conjugate cleaves DNA near the binding site in the presence of copper ions and free o-bromobenzoic acid. The efficacy and specificity of DNA cleavage by this conjugate and other oligonucleotide conjugates bearing tetracarboxyphthalocyanine CoII and bleomycin A5 as reactive groups were compared. |
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| Keywords: | oligonucleotide conjugates DNA cleavage metal complexes triplex DNA |
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