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| Crystallization and Preliminary Crystallographic Studies of Luffaculin 1,a Ribosome-inactivating Protein from the Seeds of Luffa Acutangula | |
| 作者姓名： | HOU Xiao-Mina b CHEN Ming-Huanga② XIE Jie-Mingc YE Xiao-Minga ZHAO Geng-Xianga b YANG Fenga b CHEN Li-Qingd HUANG Ming-Donga a |
| 作者单位： | HOU Xiao-Mina，b CHEN Ming-Huanga② XIE Jie-Mingc YE Xiao-Minga ZHAO Geng-Xianga，b YANG Fenga，b CHEN Li-Qingd HUANG Ming-Donga a(State Key Laboratory of Structural Chemistry，Fujian Institute of Research on the Structure of Matter，The Chinese Academy of Sciences，Fuzhou，Fujian 350002，China) b(Graduate School of the Chinese Academy of Sciences，Beijing 10039，China) c(Fujian Medical University，Fuzhou 350004，China) d(Laboratory for Structural Biology，Department of Chemistry，Graduate Programs of Biotechnology，Chemistry and Materials Science，University of Alabama in Huntsville，Huntsville，AL 35899，USA) |
| 基金项目： | 国家自然科学基金;福建省自然科学基金 |
| 摘 要： | 1 INTRODUCTION The ribosome-inactivating proteins (RIPs) are RNA N-glycosidases1, 2] which inactivate ribosomes by cleaving a single N–C glycosidic bond between adenine and ribose at A4324 in the 28s rRNA of rat. Because of the removal of one adenine from rRNA, elongation factorⅡ(EF-2) can not bind to the 60S subunit, so RIPs can arrest protein synthesis. RIPs can be classified into three types based on the struc- tures of genes and mature proteins3]. Type 1 RIPs with molec… |
Crystallization and Preliminary Crystallographic Studies of Luffaculin 1, a Ribosome-inactivating Protein from the Seeds of Luffa Acutangula |
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| HOU Xiao-Min,CHEN Ming-Huang,XIE Jie-Ming,YE Xiao-Ming,ZHAO Geng-Xiang,YANG Feng,CHEN Li-Qing,HUANG Ming-Dong.Crystallization and Preliminary Crystallographic Studies of Luffaculin 1,a Ribosome-inactivating Protein from the Seeds of Luffa Acutangula[J].Chinese Journal of Structural Chemistry,2006,25(9):1035-1038. | |
| Authors: | HOU Xiao-Min CHEN Ming-Huang XIE Jie-Ming YE Xiao-Ming ZHAO Geng-Xiang YANG Feng CHEN Li-Qing HUANG Ming-Dong |
| Institution: | 1. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, The Chinese Academy of Sciences, Fuzhou, Fujian 350002, China;Graduate School of the Chinese Academy of Sciences, Beijing 10039, China 2. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, The Chinese Academy of Sciences, Fuzhou, Fujian 350002, China 3. Fujian Medical University, Fuzhou 350004, China 4. Laboratory for Structural Biology, Department of Chemistry, Graduate Programs of Biotechnology,Chemistry and Materials Science, University of Alabama in Huntsville, Huntsville, AL 35899, USA |
| Abstract: | Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type 1 ribosome-inactivating proteins (RIPs). It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813,c= 83.571 (A), α = 89.068, β = 80.009 and γ = 72.143°, and has two molecules per asymmetric unit.X-ray data have been collected to be 1.4 (A) using a synchrotron source. |
| Keywords: | Luffa acutangula luffaculin 1 ribosome-inactivating proteins crystallization |
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