| 豆薯种子中一种具有抗植物病毒活性的蛋白质的初步晶体学研究 |
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| 引用本文： | 蔡建华,林玉娟,叶晓明,陈明晃,宋立军.豆薯种子中一种具有抗植物病毒活性的蛋白质的初步晶体学研究[J].结构化学,2001,20(2):149-150. |
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| 作者姓名： | 蔡建华 林玉娟 叶晓明 陈明晃 宋立军 |
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| 作者单位： | 中国科学院福建物质结构研究所 |
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| 基金项目： | 结构化学国家重点实验室基金，福建省自然科学基金 |
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| 摘 要： | 从豆科植物豆薯 (Pachyrrhizuserosus,Leguminosae)的种子中分离纯化了一种具有抗植物病毒活性的蛋白质 ,分子量约为 1 5kD。在 2 0 % (w/v)PEG60 0 0 ,0 .1mol/L磷酸盐缓冲液 (pH7.4)条件下得到了可供衍射用的单晶 ,在MarResearchIP上进行了衍射数据收集 ,其衍射能力达 2 .3 。经MarDenzo软件包处理 ,确定其空间群为P2 1 2 1 2 1 ,晶胞参数为 :a =2 8.80 8,b =32 .840 ,c=1 40 .397 。根据Matthews常数估算 ,每个不对称单元含 1个分子。
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| 关 键 词： | 豆薯种子 抗植物病毒活性 晶体生长 初步晶体学 |
Preliminary Crystallographic Study of a Protein with Anti-plant Virus Activity from the Seeds of Pachyrrhizus Erosus |
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| CAI Jian\|Hua\ LIN Yu\|Juan\ YE Xiao\|Ming\ CHEN Ming\|Huang\ SONG Li\|Jun.Preliminary Crystallographic Study of a Protein with Anti-plant Virus Activity from the Seeds of Pachyrrhizus Erosus[J].Chinese Journal of Structural Chemistry,2001,20(2):149-150. |
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| Authors: | CAI Jian\|Hua\ LIN Yu\|Juan\ YE Xiao\|Ming\ CHEN Ming\|Huang\ SONG Li\|Jun |
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| Abstract: | From the seeds of pachyrrhizus erosus, leguminosae, a proteinwith molecular weight of 15kD, which possesses anti-plant virus activity, has been isolated and purified. Single crystals suitable for X-ray diffraction were obtained by 20% PEG6000 and 0.1mol/L phosphate buffer. The diffraction data of the crystal were collected on a MarResearch IP, and the data at 2.3 Å resolution have been processed by using the Denzo and Scalepack software packages. Space group was determined as P212121, with cell parameters a=28.808, b=32.840 and c=140.397 Å. According to the estimation of Matthews constants, there is one molecule in an asymmetric unit. |
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| Keywords: | pachyrrhizus erosus anti-plant virus crystal growth crystallography |
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