Temperature effect on HPLC retention of PCBs on porous graphitic carbon |
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Authors: | M C Pietrogrande A Benvenuti F Dondi |
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Institution: | (1) Department of Chemistry, University of Ferrara, Via L. Borsari, 46, 44100 Ferrara, Italy |
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Abstract: | Summary The effect of column temperature on solute retention was investigated for 10 PCBs with different degrees of planarity (degree
of ortho position substitution). The variation of retention values (lnk) with temperature was measured using two solvents, n-hexane and dichloromethane, as mobile phase: retention decreases as
temperature increases. The relationship between lnk values and temperature (1/T) exhibits a good linearity. The ΔH
0 values calculated are in the −2 to −5 kcal mole−
1 range: they are very similar for compounds with the same degree of ortho-substitution and are significantly higher for non-ortho
substituted congeners. High temperature significantly improves separation efficiency (plate number significantly increases):
this effect is the dominant factor controlling chromatographic resolution, which improves with increasing temperature. Operating
at 40 °C, an efficient separation of planar PCBs was obtained with low solvent consumption. |
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Keywords: | Column liquid chromatography Porous graphitic carbon Temperature effect on retention Polychlorobyphenyls |
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