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Quantitative Characterization of Bovine Serum Albumin, α-Lactalbumin and β-Lactoglobulin in Commercial Whey Sample by RP-LC |
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| Authors: | Goutam Mukhopadhyay Jasmina Khanam Arunabha Nanda Arindam Basu Sarkar |
| Institution: | 1. B.C.D.A College of Pharmacy and Technology, 78 Jessore Road (S) Hridaypur, Barasat, Kolkata, 700127, India 2. Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India 3. School of Pharmacy, The University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA |
| Abstract: | A simple, sensitive and precise reverse phase liquid chromatographic method has been developed and validated for quantification of bovine serum albumin (BSA), α-lactalbumin (α-La) and β–lactoglobulin (β-Lg) that are removed from whey waste by foam fractionation method. The data is reproducible over a wide concentration range. This optimized method allowed analysis of BSA, α-La, β-Lg in a mixture within 5 min and could be applied to the analysis of a variety of commercial and laboratory whey products within a short time. |
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