Evaluation of a sensitive HPLC method for the determination of Malondialdehyde, and application of the method to different biological materials |
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Authors: | G A Khoschsorur B M Winklhofer-Roob H Rabl Th Auer Z Peng R J Schaur |
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Institution: | (1) Department of Clinical Chemistry and Laboratory Medicine (BL I), Surgery Clinic, University of Graz, 8036 Graz, Austria;(2) Institute of Molecular Biology, Biochemistry and Microbiology, University of Graz, 8036 Graz, Austria;(3) Department of General Surgery, University of Graz, 8036 Graz, Austria |
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Abstract: | Summary Reactive oxygen species (ROS), important mediators of cell and tissue injury during inflammation, are produced by several
types of inflammatory cells. The formation of ROS can be monitored by detection of lipid peroxidation products. The extremely
broad spectrum of biological effects of aldehydic lipid peroxidation products has necessitated the development of a technique
that enables the sensitive routine quantitation of aldehydes formed in biological materials. Malondialdehyde (MDA) is a by-product
of enzymatic eicosanoid formation and an end-product of nonenzymatic peroxidation of polyunsaturated fatty acids with three
or more bisallylic double bonds. The determination of the thiobarbituric acid derivative of MDA (TBA-MDA) is a widely used
method for estimating overall lipid peroxidation. We describe a rapid, isocratic, simple, and sensitive high-performance liquid
chromatographic (HPLC) method with spectrofluorimetric detection for measurement of MDA-TBA in human biological samples such
as plasma, urine, wound secretions, amniotic fluid, sputum and tissue samples. By use of this method, picomole quantities
of MDA can be readily and specifically detected in different biological materials. Coefficients of variation of repeated MDA-TBA
assays were 4.4% within run and 6.9% from run to run. Reference values are given for a variety of human body fluids and for
rat tissues. |
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Keywords: | Column liquid chromatography Spectrofluorimetry Thiobarbituric acid Malondialdehyde Human body fluids and tissues |
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