Simplified Solid-Phase Extraction Procedure and Liquid Chromatographic Determination of Celecoxib in Rat Serum |
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Authors: | M H Guermouche A Gharbi |
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Institution: | (1) Faculté de Chimie, USTHB, B.P. n°32, El-Alia, Bab-Ezzouar, Alger, Algeria;(2) LNC, Département de Pharmacie, Faculté mixte de Medecine et Pharmacie, Université dAlger, 2, rue Didouche Mourad, Alger, Algeria |
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Abstract: | A simplified solid phase extraction method, eliminating a preliminary protein precipitation has been developed for the determination of celecoxib in rat plasma. The technique included a solid phase extraction of the serum samples on a poly (divinylbenzene-co-N-vinylpyrrolidone)] sorbent. After conditioning, the cartridge was loaded with 0.5 mL of acidified serum containing internal standard. Elution was made with 1 mL of a mixture of acetonitrile and methanol (1/1, v/v). After evaporation of the eluate to dryness and reconstitution with methanol, the samples were analyzed on an octadecyl bonded phase with several mobile phases containing acetonitrile and a phosphate buffer. Detection was carried out using a Photodiode Array Detector. Full validation of the proposed method was provided (linearity range: 0.01–2 mg. L–1, average extraction efficiency: 92.4%; average intra-day variability: 4.6% with an accuracy of 94.8%; average interday variability: 5% with an accuracy of 95.3%, limit of detection: 0.005 mg. L–1, limit of quantification: 0.002 mg. L–1). The proposed method was successfully utilised to quantify celecoxib in rat plasma for a pharmacokinetic study.Revised: 26 January and 23 April 2004 |
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Keywords: | Column liquid chromatography Solid phase extraction Photodiode array detector Celecoxib in rat serum |
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