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Enantiomeric Composition Tests of Ketoprofen in Equine Plasma and Urine as Diastereomeric (S)-(-)-1-Phenylethylamides by Achiral GC–MS |
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| Authors: | J Yu G Lee Y Jeong Y H Ahn M B Hu S J Kim K R Kim M J Paik |
| Institution: | 1. Racing Laboratory, Korea Racing Authority, Gwacheon, 427-711, Republic of Korea 2. Biometabolite Analysis Laboratory, College of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Republic of Korea 3. Institute for Neuroregeneration and Stem Cell Research, School of Medicine, Ajou University, Suwon, 443-721, Republic of Korea 4. Department of Molecular Science and Technology, School of Medicine, Ajou University, Suwon, 443-721, Republic of Korea 5. Institute for Medical Science, School of Medicine, Ajou University, Suwon, 443-721, Republic of Korea 6. Department of Neurosurgery, School of Medicine, Ajou University, Suwon, 443-721, Republic of Korea |
| Abstract: | The enantiomeric composition of ketoprofen in equine plasma and urine after administration of a commercial racemic ketoprofen product were determined as diastereomeric (S)-(-)-1-phenylethylamidation by achiral gas chromatography–mass spectrometry in selected ion monitoring mode. This method showed linearity (r ≥ 0.9986) over the range tested (5.0–5,000 ng), with acceptable precision (% RSD ≤ 9.5) and accuracy (% RE = ?3.7–7.3). The ratio of (S)-ketoprofen in plasma after 6.0 h and in urine after 71.0 h increased progressively to final values of 67.3 ± 0.1 and 91.9 ± 2.2%, respectively, attributable to the inversion of (R)-ketoprofen to (S)-ketoprofen. |
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