GC determination of nicotine in subcutaneous adipose tissue obtained by minimal trauma tissue biopsy |
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Authors: | B X Mayer M Brunner M Müller H Mascher H G Eichler |
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Institution: | 1. Department of Clinical Pharmacology, Division of Clinical Pharmacokinetics, University Hospital of Vienna, W?hringer Gürtel 18-20, 1090, Vienna, Austria 2. Pharm. Analyt. Laboratory GmbH, Ferdinand-Pichler-Gasse 2, 2500, Baden, Austria
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Abstract: | Summary Nicotine has been analyzed by gas chromatography nitrogen-phosphorus detection in tissue samples obtained by repeated minimal
trauma tissue biopsies from human subcutaneous adipose tissue. For sample preparation, a single extraction step of the tissue
samples with chloroform was performed at 30–45°C. Calibration curves generated with spiked porcine subcutaneous adipose tissue
were linear over a concentration range from 0.20 to 100 μg g−1, therefore, the limit of quantification was fixed at 0.20 μg g−1. The limit of detection was found to be 0.05 μg g−1 adipose tissue. The recovery of nicotinespiked porcine subcutaneous adipose tissue by chloroform extraction was 100±8%. The
performance of the assay was not affected by the complex lipid matrix. The method was employed for analysis in a clinical
study on nicotine penetration from a transdermal delivery system through the skin of moderate smokers.
Presented at Balaton Symposium on High Performance Separation Methods, Siófok, Hungary, September 1–3, 1999. |
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Keywords: | Gas chromatography Nitrogen-phosphorus detection Nicotine Subcutaneous adipose tissue Tissue biopsy |
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