Simultaneous Analysis of a Mixture of Iridoids in Water Extracts of Plantago asiatica L. by LC |
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Authors: | Bong-Hyun Kim Nam-Kyung Lee and Il-Moo Chang |
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Institution: | (1) College of Pharmacy, Natural Products Research Institute, Seoul National University, Seoul, 151-742, Korea; |
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Abstract: | A simple liquid chromatographic method using an ultraviolet detector was developed for the simultaneous quantification of
a mixture of iridoid glycosides, catalpol, aucubin, and geniposidic acid. By adding a minute amount of trifluoroacetic acid
into the conventional aqueous acetonitrile mobile phase, the unstable resolution arising from the tautomeric rearrangements
in the carboxylic group at the 11 position of geniposidic acid could be solved, resulting in a clear separation profile of
the catalpol, aucubin, and geniposidic acid peaks. We were able to quantify the contents of catalpol, aucubin, and geniposidic
acid contained in the leaves and seeds of P. asiatica. |
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Keywords: | |
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