Dendronized Anionic Gold Nanoparticles: Synthesis,Characterization, and Antiviral Activity |
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| Authors: | Cornelia E Peña‐González Pilar García‐Broncano Prof M Francesca Ottaviani Dr Michela Cangiotti Dr Alberto Fattori Margarita Hierro‐Oliva Prof M Luisa González‐Martín Dr Jorge Pérez‐Serrano Dr Rafael Gómez Prof M Ángeles Muñoz‐Fernández Dr Javier Sánchez‐Nieves Dr F Javier de la Mata |
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| Institution: | 1. Dpto. de Química Orgánica y Química Inorgánica, Edificio de Farmacia, Universidad de Alcalá, Alcalá de Henares (Madrid), Spain;2. Unidad de Infección Viral e Inmunidad, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain;3. Laboratorio de InmunoBiología Molecular, Hospital General Universitario Gregorio Mara?ón, Madrid, Spain;4. Instituto de Investigación Sanitaria Gregorio Mara?ón, Madrid, Spain;5. Spanish HIV HGM BioBank, Madrid, Spain;6. Department of Earth, Life and Environment Sciences, University of Urbino, Urbino, Italy;7. Departamento de Física Aplicada, Facultad de Ciencias, Campus Universitario, Universidad de Extremadura, Badajoz, Spain;8. Networking Research Center for Bioengineering Biomaterials and Nanomedicine (CIBER-BBN), Instituto de Salud Carlos III, Madrid, Spain;9. Departamento de Biomedicina y Biotecnología, Edificio de Farmacia, Campus Universitario, Universidad de Alcalá, Alcalá de Henares, Spain |
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| Abstract: | Anionic carbosilane dendrons decorated with sulfonate functions and one thiol moiety at the focal point have been used to synthesize water‐soluble gold nanoparticles (AuNPs) through the direct reaction of dendrons, gold precursor, and reducing agent in water, and also through a place‐exchange reaction. These nanoparticles have been characterized by NMR spectroscopy, TEM, thermogravimetric analysis, X‐ray photoelectron spectroscopy (XPS), UV/Vis spectroscopy, elemental analysis, and zeta‐potential measurements. The interacting ability of the anionic sulfonate functions was investigated by EPR spectroscopy with copper(II) as a probe. Different structures and conformations of the AuNPs modulate the availability of sulfonate and thiol groups for complexation by copper(II). Toxicity assays of AuNPs showed that those produced through direct reaction were less toxic than those obtained by ligand exchange. Inhibition of HIV‐1 infection was higher in the case of dendronized AuNPs than in dendrons. |
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| Keywords: | antiviral agents cytotoxicity dendrimers nanoparticles structure– activity relationships |
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