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Durable Mesenchymal Stem Cell Labelling by Using Polyhedral Superparamagnetic Iron Oxide Nanoparticles |
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| Authors: | Hao‐Hao Wang Yi‐Xiang J Wang Prof Ken Cham‐Fai Leung Prof Doris W T Au Prof Shouhu Xuan Dr Chun‐Pong Chak Simon K M Lee Dr Hui Sheng Dr Ge Zhang Dr Ling Qin Prof James F Griffith Prof Anil T Ahuja Prof |
| Institution: | 1. Department of Diagnostic Radiology and Organ Imaging, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (China), Fax: (+852) 26360012;2. Department of Oncological Surgery, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province (China);3. Center of Novel Functional Molecules, Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (China), Fax: (+852) 26035057;4. Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong SAR (China);5. Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR (China) |
| Abstract: | Small polyhedral superparamagnetic iron oxide (SPIO) nanoparticles (<10 nm) coated with a thin layer of silica were prepared (SPIO@SiO2 and SPIO@SiO2‐NH2). Surface modification of the small polyhedral silica‐coated SPIO nanoparticles with amines led to substantially higher mesenchymal stem cell (MSC) labelling efficiency without the use of additional transfecting agents. Therefore, amine surface‐modified nanoparticles (SPIO@ SiO2‐NH2) appeared to be the preferred candidate for MSC labelling. In vitro studies demonstrated that controlled labelling of SPIO@SiO2 and SPIO@SiO2‐NH2 did not cause MSC death or proliferation inhibition. MSCs labelled with SPIO@SiO2‐NH2 nanoparticles retained differentiation potential and showed osteogenic, adipogenic and chondrogenic differentiations. The noncytotoxic polyhedral SPIO@SiO2‐NH2 nanoparticle‐labelled MSCs were successfully implanted in rabbit brain and erector spinae muscle, and demonstrated long‐lasting, durable MRI labelling efficacy after 8–12 weeks. |
| Keywords: | contrast agents diagnostic agents iron magnetic properties nanoparticles |