| 紫外圆二色光谱预测蛋白质结构的研究方法 |
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| 引用本文： | 黄汉昌,姜招峰,朱宏吉.紫外圆二色光谱预测蛋白质结构的研究方法[J].化学通报,2007,70(7):501-506. |
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| 作者姓名： | 黄汉昌 姜招峰 朱宏吉 |
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| 作者单位： | 北京联合大学应用文理学院生物活性物质与功能食品北京市重点实验室 北京100083(黄汉昌，姜招峰)，天津大学化工学院 天津300072(朱宏吉) |
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| 基金项目： | 北京市教委和北京市自然基金资助项目(KZ200311417015) |
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| 摘 要： | 介绍了蛋白质紫外圆二色性(CD)产生的原理及其与蛋白质结构的关系。评述了用远紫外CD预测蛋白质二级结构的方法原理、参考蛋白、拟合算法和拟合程序,以及方法存在的问题。近紫外CD与蛋白质的三级结构密切相关,近紫外蛋白质CD反映芳香氨基酸残基、二硫键等微环境的变化,表征着丰富的蛋白质三级结构的信息。
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| 关 键 词： | 圆二色 蛋白质结构 紫外光谱 |
| 修稿时间： | 2006-12-04 |
The Methods of Protein Conformation Predicted by UV-Circular Dichroism |
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| Huang Hanchang,Jiang Zhaofeng,Zhu Hongji.The Methods of Protein Conformation Predicted by UV-Circular Dichroism[J].Chemistry,2007,70(7):501-506. |
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| Authors: | Huang Hanchang Jiang Zhaofeng Zhu Hongji |
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| Institution: | 1.Beijing Union University Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing 100083 ; 2.School of chemical engineering and technology, Tianjin University, Tianjin 300072 |
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| Abstract: | The principle of protein circular dichroism(CD) and the relationship between protein structures and its CD are introduced in this article.The methods,including principle,reference proteins,fitting algorithm and its program,of protein secondary structures predicted by far ultraviolet(UV) CD are reviewed.The proteins near UV CD spectra contain the information of the aromatic amino acids and disulfide group which are hypersensitive to environmental changes,and they imply the protein tertiary structures information. |
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| Keywords: | Circular dichroism Protein secondary structure Ultraviolet spectra |
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