| 加速溶剂萃取-凝胶色谱净化高效液相色谱法测定动物源性食品中的胆固醇 |
| |
| 引用本文： | 张晓林,曲世超,黄大亮,赵守成,郭雅琳,刘莹.加速溶剂萃取-凝胶色谱净化高效液相色谱法测定动物源性食品中的胆固醇[J].化学通报,2015,78(3):281-284. |
| |
| 作者姓名： | 张晓林 曲世超 黄大亮 赵守成 郭雅琳 刘莹 |
| |
| 作者单位： | 大连出入境检验检疫局 大连庄河 116400 |
| |
| 摘 要： | 采用加速溶剂萃取-凝胶色谱净化建立了动物源性食品中胆固醇检测的高效液相色谱方法。结果表明,胆固醇浓度在1.0~40.0mg/L范围内线性关系良好,加标回收率为89.1%~99.8%,相对标准偏差为3.1%~7.8%。该方法准确、灵敏度高,适用于富含脂类的动物源性食品中胆固醇的检测。
|
| 关 键 词： | 加速溶剂萃取 凝胶色谱净化 胆固醇 液相色谱 |
| 收稿时间： | 4/3/2014 12:00:00 AM |
| 修稿时间： | 2014/6/16 0:00:00 |
Determination of cholesterol in animal originated food using accelerated solvent extraction and gel permeation chromatography purification with high performance liquid chromatography |
| |
| zhang xiao lin,qushichao,huangdaliang,zhaoshoucheng,guoyalin and liuying.Determination of cholesterol in animal originated food using accelerated solvent extraction and gel permeation chromatography purification with high performance liquid chromatography[J].Chemistry,2015,78(3):281-284. |
| |
| Authors: | zhang xiao lin qushichao huangdaliang zhaoshoucheng guoyalin and liuying |
| |
| Institution: | Zhang Xiaolin;Qu Shichao;Huang Daliang;Zhao Shoucheng;Guo Yalin;Liu Ying;Technology Center of Dalian Entry-Exit Inspection and Quarantine Bureau; |
| |
| Abstract: | A method for the determination of cholesterol in animal originated food has been developed. The target analytes were extracted with hexane/isopropyanol(v/v, 3/2) by accelerated solvent extraction(ASE),and then purified by gel permeation chromatography(GPC). The results showed a good linear correlation in the range of 1.0-40.0mg/L, and the average recoveries were 89.1% - 99.8% , with the relative standard deviations being from 3.1% to 7.8%. The method shows advantages in both accuracy and sensitivity , and is suitable for the determination of cholesterol in animal originated food. |
| |
| Keywords: | accelerated solvent extraction gel permeation chromatography cholesterol high performance liquid chromatography |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《化学通报》浏览原始摘要信息 |
| 点击此处可从《化学通报》下载免费的PDF全文 |
|
