用于一氧化氮检测的小分子荧光探针研究进展

生物小分子NO以其重要的生理学和病理学作用受到科学家们的广泛关注。高选择性、高灵敏度、低毒性NO分子荧光探针的设计和开发,在环境检测、食品安全及人体内NO检测等领域具有重要意义。本文以小分子荧光探针对NO的识别机制为主线,从唑环的形成、螺内酰胺开环、还原脱氨、二氢吡啶的芳构化、NO与金属络合物的反应、与非金属Se的反应和亚硝胺的形成出发,综述了近年来NO小分子荧光探针的研究进展。对NO探针设计及其识别性能研究方面的工作进行了总结,并讨论了NO荧光探针今后的设计思路和重点研究方向。

Progress in Small Molecular Fluorescent Probes for Nitric Oxide Detection

The biological small molecule NO has received extensive attention from scientists for its important physiological and pathological effects. Studies have found that NO not only promotes vasodilation in the human body, but also has specific associations with the hypoxic regions of tumor cells. On this basis, in recent years, researchers have made great progress in the determination of their concentration using fluorescent probes. The design and development of high-selectivity, ultra-sensitive, low-toxicity NO molecular fluorescent probes are of great significance in environmental testing, food safety, and NO detection in humans. In this paper, the recognition mechanism of NO by small molecule fluorescent probes is the main line, from the formation of azole ring, spironolactam ring opening, reductive deamination, aromatization of dihydropyridine, NO and metal complex In the reaction, the reaction with non-metal Se and the formation of nitrosamines, the research progress of NO small molecule fluorescent probes in recent years is reviewed. In recent years, the work of NO probe design and its recognition performance research has been systematically summarized, and the future design ideas and key research directions of NO fluorescence probes are discussed.