| 基于氧荧光猝灭速率法的生化需氧量检测 |
| |
| 引用本文： | 肖来龙,辛玲玲,赵丽,章丽燕,陈曦,王小如.基于氧荧光猝灭速率法的生化需氧量检测[J].化学通报,2006,69(7):508-513. |
| |
| 作者姓名： | 肖来龙 辛玲玲 赵丽 章丽燕 陈曦 王小如 |
| |
| 作者单位： | 厦门大学化学化工学院化学系现代分析科学教育部重点实验室，厦门大学化学化工学院化学系现代分析科学教育部重点实验室，厦门大学化学化工学院化学系现代分析科学教育部重点实验室，厦门大学化学化工学院化学系现代分析科学教育部重点实验室，厦门大学化学化工学院化学系现代分析科学教育部重点实验室，厦门大学化学化工学院化学系现代分析科学教育部重点实验室 龙岩学院化学与材料工程系，福建龙岩364000，厦门大学近海海洋环境科学国家重点实验室，厦门361005 |
| |
| 基金项目： | 国家科技攻关项目;福建省自然科学基金 |
| |
| 摘 要： | 利用氧荧光猝灭速率的方法,结合自行构建的BOD光纤传感装置进行海水中生化需氧量(BOD)含量检测。考察了四种筛选的海洋耗氧菌种在四甲基硅氧烷(TMOS)、二甲基二甲氧基硅烷(Di Me-DMOS)和聚乙烯醇(PVA)包埋固定情况下,对不同浓度的葡萄糖-谷氨酸(GGA)标准溶液的荧光响应情况。BOD敏感膜的荧光响应在0·2~30mg/L浓度范围内呈良好的线性关系,对2mg/L标准溶液测定的相对标准偏差为2·5%(n=6),响应时间(t95%)为4·0min,BOD敏感膜使用寿命大于12个月。实际海水样品检测表明,利用BOD敏感膜检测得到的结果与国标BOD5方法之间存在较好的一致性。
|
| 关 键 词： | 荧光猝灭速率 生化需氧量(BOD) 光纤传感器 |
| 收稿时间： | 2005-10-13 |
| 修稿时间： | 2005-10-132006-01-23 |
BOD Measurement Based on Oxygen Fluorescent Quenching Rate Method |
| |
| Xiao Lailong,Xin Lingling,Zhao Li,Zhang Liyan,Chen Xi,Wang Xiaoru.BOD Measurement Based on Oxygen Fluorescent Quenching Rate Method[J].Chemistry,2006,69(7):508-513. |
| |
| Authors: | Xiao Lailong Xin Lingling Zhao Li Zhang Liyan Chen Xi Wang Xiaoru |
| |
| Institution: | 1. The Key Laboratory of Analytical Sciences of Ministry of Education, Department of Chemistry, Xiamen; 2.State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005; 3. Department of Chemical and Material Engineering, Longyan College, Longyan 364000 |
| |
| Abstract: | Based on oxygen fluorescent quenching rate method,a BOD optical sensing film immobilized four kinds of microorganisms from seawater in an Ormosils-PVA matrix was applied for BOD measurement of seawater. The responding characteristic of fluorescent quenching is researched. The BOD sensing film contributed a good linear relationship between concentration and fluorescent response in the concentration range of 0.2 to 30mg/L. Measurements were taken for 4min followed by 10min recovery time in 2mg/L glucose/glutamate (GGA) BOD standard solution. Some other experimental conditions, such as pH, measurement temperature, the concentration of sodium chloride, were studied. BOD values estimated by this optical BOD sensing film correlate well with those determined by the conventional BOD_5 method for seawater samples. |
| |
| Keywords: | Fluorescent quenching rate BOD sensor Optical fiber |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
