| 基于菁染料响应G-四链体结构变化的半胱氨酸检测探针 |
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| 引用本文： | 田佳琦,陈月荣,刘铭,张艳华,孙旭东,付尧,张秀凤,石磊.基于菁染料响应G-四链体结构变化的半胱氨酸检测探针[J].化学通报,2020,83(11):1025-1030. |
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| 作者姓名： | 田佳琦 陈月荣 刘铭 张艳华 孙旭东 付尧 张秀凤 石磊 |
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| 作者单位： | 华北理工大学化学工程学院 唐山 063210;华北理工大学化学工程学院 唐山 063210;华北理工大学化学工程学院 唐山 063210;华北理工大学化学工程学院 唐山 063210;华北理工大学化学工程学院 唐山 063210;华北理工大学化学工程学院 唐山 063210;华北理工大学化学工程学院 唐山 063210;华北理工大学化学工程学院 唐山 063210 |
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| 基金项目： | 华北理工大学大学生创新创业训练计划项目(X2018166)和国家自然科学基金项目(21807034)资助 |
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| 摘 要： | 半胱氨酸是生物体中起着重要作用的还原性氨基酸,其在体内的含量变化可能会诱发机体发生多种病变。因此高选择性、高灵敏度和低成本的半胱氨酸检测技术具有重要意义。目前,高效的半胱氨酸检测方法有毛细管电泳、质谱、高效液相色谱和表面增强拉曼散射等,这些方法往往需要复杂的样品制备和精细的实验仪器,可能会限制其在半胱氨酸检测中的应用。本文利用富含鸟嘌呤的DNA链序列在钾、钠等金属离子诱导下形成对汞离子产生特殊响应的二级结构,而菁染料能够对DNA结构进行识别,并由此引起其超分子聚集形式的改变,致使其紫外和可见光谱性质随之变化。最终以Hg2+调控G-四链体与菁染料(ETC)组建的传感器,实现对溶液体系中半胱氨酸高选择性的快速可视化检测。
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| 关 键 词： | DNAG-四链体 菁染料 巯基蛋白 半胱氨酸 紫外光谱 |
| 收稿时间： | 2020/4/11 0:00:00 |
| 修稿时间： | 2020/6/9 0:00:00 |
A Cysteine Detection Probe Based on Regulating Cyanine Dye Transformation by G-quardruplex |
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| Tian Jiaqi,Chen Yuerong,Liu Ming,Zhang Yanhu,Sun Xudong,Fu Yao,Zhang Xiufeng,Shi Lei.A Cysteine Detection Probe Based on Regulating Cyanine Dye Transformation by G-quardruplex[J].Chemistry,2020,83(11):1025-1030. |
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| Authors: | Tian Jiaqi Chen Yuerong Liu Ming Zhang Yanhu Sun Xudong Fu Yao Zhang Xiufeng Shi Lei |
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| Institution: | School of Chemical Engineering,North China University of Science and Technology,Tangshan Hebei 063210;China,School of Chemical Engineering,North China University of Science and Technology,Tangshan Hebei 063210;China,School of Chemical Engineering,North China University of Science and Technology,Tangshan Hebei 063210;China,School of Chemical Engineering,North China University of Science and Technology,Tangshan Hebei 063210;China,School of Chemical Engineering,North China University of Science and Technology,Tangshan Hebei 063210;China,School of Chemical Engineering,North China University of Science and Technology,Tangshan Hebei 063210;China,School of Chemical Engineering,North China University of Science and Technology,Tangshan Hebei 063210;China,School of Chemical Engineering,North China University of Science and Technology,Tangshan Hebei 063210;China |
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| Abstract: | Cysteine is a reducing amino acid that plays an important role in organisms, and changes its content in the body may induce a variety of lesions. Therefore, cysteine detection technology with high selectivity, high sensitivity and low cost is of great significance. At present, high-efficiency cysteine detection methods include capillary electrophoresis, mass spectrometry, high-performance liquid chromatography, and surface-enhanced Raman scattering. These methods often require complex sample preparation and sophisticated experimental equipment, which may limit their use in application of acid detection. In this paper, a guanine-rich DNA sequence is induced by potassium and sodium metal ions to form a secondary structure that has a special response to mercury ion. The cyanine dye can recognize the DNA structure. The transformation of the supramolecular orm causes its UV and visible spectral properties to change accordingly. Finally, the sensor composed of Hg2+ regulating G-quadruplex and cyanine dye is utilized in the rapid visual and highly selective detection of cysteine in the solution system. |
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| Keywords: | DNA G-quadruplex cyanine dyes thiol protein cysteine UV spectrum |
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