| 甲基橙皮苷和人血清白蛋白相互作用的光谱研究 |
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| 引用本文： | 李金华,王素敏,朱金花.甲基橙皮苷和人血清白蛋白相互作用的光谱研究[J].化学研究,2013(2):176-179. |
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| 作者姓名： | 李金华 王素敏 朱金花 |
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| 作者单位： | 西安工业大学材料科学与化工学院;河南大学化学化工学院 |
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| 基金项目： | 陕西省教育厅专项科研计划项目(2010JK609) |
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| 摘 要： | 利用荧光光谱法和红外光谱法研究了甲基橙皮苷(MH)与人血清白蛋白(HSA)的相互作用.结果表明,MH对HSA的荧光有较强的猝灭作用.在296、303、310K温度下,MH与HSA相互作用的结合常数分别为1.77×104,2.65×104,3.53×104 L·mol-1.热力学分析结果表明,MH与HSA之间的结合过程是吸热的并且是自发的;作用力以疏水作用为主,并伴随氢键作用.
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| 关 键 词： | 甲基橙皮苷 人血清白蛋白 相互作用 荧光光谱 红外光谱 |
Interaction between methyl hesperidin and human serum albumin studied by spectrometric methods |
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| LI Jin-hua,WANG Su-min,ZHU Jin-hua.Interaction between methyl hesperidin and human serum albumin studied by spectrometric methods[J].Chemical Research,2013(2):176-179. |
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| Authors: | LI Jin-hua WANG Su-min ZHU Jin-hua |
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| Institution: | 2(School of Materials and Chemical Engineering,Xi’an Technological University,Xi’an 710032,Shaanxi,China; 2.College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004,Henan,China) |
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| Abstract: | The interaction between methyl hesperidin （MH） and human serum albumin （HSA） under simulative physiological conditions was studied by spectroscopic methods including fluo- rescence quenching technology and Fourier transform infrared （FT-IR） spectrometry. Results reveal that MH can well quench the intrinsic fluorescence of HSA. The binding constants at 296, 303, and 310 K are 1.77×104 , 2.65×104 , and 3.53×104 L .mol-1 , respectively. Ther- modynamic analyses show that the binding process between MH and HSA is endothermic and spontaneous, and the interaction is dominated by hydrophobic interaction, accompanied by hy- drogen bonding. |
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| Keywords: | methyl hesperidin human serum albumin interaction fluorescence spectrometry infrared spectrometry |
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