氨基酸衍生物在毛细管区带电泳下的分离研究 |
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引用本文: | 明永飞,孙玉希,尤进茂.氨基酸衍生物在毛细管区带电泳下的分离研究[J].化学研究,2003,14(3):9-12. |
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作者姓名: | 明永飞 孙玉希 尤进茂 |
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作者单位: | 曲阜师范大学,化学科学学院,山东,曲阜,273165 |
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基金项目: | 国家自然科学基金资助项目(No.20075016). |
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摘 要: | 采用新合成的荧光试剂咔唑-9-乙基氯甲酸酯作为柱前衍生试剂,利用毛细管区带电泳法对衍生氨基酸进行分离,考察了该试剂用于毛细管区带电泳法进行氨基酸分离的关键条件,实现了12种氨基酸的快速基线分离.
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关 键 词: | 毛细管区带电泳 氨基酸 衍生化 |
文章编号: | 1008-1011(2003)03-0009-04 |
Separation of Amino Acids by Capillary Zone Electrophoresis Using 9-(2-Carbazole) Ethyl Chloroformate as Derivatization Agent |
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Abstract: | A simple and rapid method for the separation of amino acids by capillary zone electrophoresis using 9 (2 carbazole)ethyl chloroformate (CEOC) as derivatization agent was developed. The effect of some key factors such as pH, temperature and concentration of buffer, on the separation of amino acid derivatives was investigated. 12 amino acids were separated with satisfactory results. |
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Keywords: | capillary zone electrophoresis amino acid derivatization |
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