| 水飞蓟素与牛血清白蛋白相互作用的荧光光谱研究 |
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| 引用本文： | 尚永辉,孙家娟,刘静.水飞蓟素与牛血清白蛋白相互作用的荧光光谱研究[J].化学研究,2011,22(2):1-3. |
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| 作者姓名： | 尚永辉 孙家娟 刘静 |
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| 作者单位： | 咸阳师范学院化学与化工系,陕西,咸阳,712000 |
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| 基金项目： | 国家自然科学基金项目(20975081); 西北大学研究生交叉学科资助项目(07YJC09); 陕西省教育厅科研计划项目(2010JK899) |
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| 摘 要： | 采用同步荧光光谱技术研究了pH=7.40的Tris-HCl缓冲体系中水飞蓟素与牛血清白蛋白(BSA)的相互作用以及水飞蓟素对BSA构象的影响.结果表明,水飞蓟素对BSA的荧光猝灭过程为静态猝灭,结合热力学参数△rH∞=-45.21 kJ·mol-1,△rSm=-61.61 J·K-1·mol-1;据此可以推断,水飞蓟素...
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| 关 键 词： | 水飞蓟素 牛血清白蛋白 相互作用 荧光光谱 |
Fluorescence spectrometric study of interaction between silymarin and bovine serum albumin |
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| SHANG Yong-hui,SUN Jia-juan,LIU Jing.Fluorescence spectrometric study of interaction between silymarin and bovine serum albumin[J].Chemical Research,2011,22(2):1-3. |
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| Authors: | SHANG Yong-hui SUN Jia-juan LIU Jing |
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| Institution: | SHANG Yong-hui,SUN Jia-juan,LIU Jing(Department of Chemistry & Chemical Engineering,Xianyang Normal College,Xianyang 712000,Shaanxi,China) |
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| Abstract: | The interaction between silymarin and bovine serum albumin（BSA） in physiological buffer solution（pH = 7.40） as well as the effect of silymarin on the conformation of BSA were studied by means of synchronous fluorescence spectrometry.It was found that silymarin quenched the fluorescence of BSA via a static quenching process,with thermodynamic parameters including enthalpy change（△rHm） and entropy change（△rSm） being-45.21 kJ·mol-1 and-61.61 J·K-1·mol-1,respectively.Thus it could be inferred that the interaction between silymarin and BSA was driven mainly by hydrogen bonding and Van der Waals forces,and the binding process was a spontaneous process. |
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| Keywords: | silymarin bovine serum albumin interaction fluorescence spectrometry |
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