| 降血脂药物中L-肉毒碱的高效液相色谱法测定 |
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| 引用本文： | 李景华,赵炎葱,韩光.降血脂药物中L-肉毒碱的高效液相色谱法测定[J].化学研究,2014(4):390-393. |
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| 作者姓名： | 李景华 赵炎葱 韩光 |
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| 作者单位： | [1]河南大学天然药物与免疫工程重点实验室,河南开封475001 [2]河南大学第一附属医院,河南开封475001 [3]河南大学药学院,河南开封475001 |
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| 基金项目： | 国家十一五重大新药创制科技重大专项子课题（2009ZX09501-025） |
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| 摘 要： | 建立了高效液相色谱法测定降血脂药物中L-肉毒碱含量的简便方法.采用Kromasil NH2柱(250mm×4.6mm,5μm),以乙腈∶KH2PO4溶液(6.81g/L,用NaOH调至pH=4.7)=65∶35为流动相,流速为1.0mL/min,检测波长为205nm.结果显示,在用高效液相色谱测试L-肉毒碱(0.4g/L)时,其注射体积在0.5~10L范围内与色谱峰面积呈良好的线性关系,相关系数为0.999 9,回收率为99.53%,RSD为0.31%.该方法操作简便、快速、有良好的准确度,可作为降血脂药物中L-肉毒碱含量的测定.
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| 关 键 词： | 降血脂药物 L-肉毒碱 HPLC 测定 |
Detemination of L-camitine in lipid-lowing drugs byhigh performance liquid chromatograph |
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| Li Jinghua,Zhao Yancong,Han Guang.Detemination of L-camitine in lipid-lowing drugs byhigh performance liquid chromatograph[J].Chemical Research,2014(4):390-393. |
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| Authors: | Li Jinghua Zhao Yancong Han Guang |
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| Institution: | 1. Key Laboratory of Natural Medicine and Immunal Engineering, Henan University, Kaifeng 475001, Henan, China; 2. The First Affiliated Hospital, Henan University, Kaifeng 475001, Henan, China ;3. Pharmacy College, Henan University; Kaifeng 475001, Henan, China) |
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| Abstract: | A high performance liquid chromatograph（HPLC）method was established to determine the content of L-camitine in lipid-lowing drugs.The separarion was performed with Kromasil NH2column（250mm×4.6mm,5μm）.The mobile phase consists of a cetonitrile：KH2PO4（6.81g/L,pH=4.7）=65∶35,and the flow rate and detection wavelength are 1.0mL/min and 205nm.Results indicate that,under the optimal conditions,the relationship between the injection volume（0.5-10μL）of L-camitine（0.4g/L）and its peak area was shown to be linear（r=0.999 9）.The recovery was 99.53%,RSD was 0.31%.The method is simple,quick and suitable for the determination of L-carnitine in lipid-lowing drugs. |
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| Keywords: | lipid-lowingdrugs L-camitine HPLC detemination |
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