Amperometric determination of NADH at a Nile blue/ordered mesoporous carbon composite electrode |
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| Authors: | Liande Zhu Ruilan Yang Xiaoyan Jiang Dongxu Yang |
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| Institution: | School of Chemistry, Northeast Normal University, No. 5268, Renmin Street, Changchun, Jilin 130024, China |
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| Abstract: | A novel amperometric NADH sensor was presented based on a Nile blue A (NB)/ordered mesoporous carbon (OMC) composite (NB/OMC) electrode. Cyclic voltammetric tests revealed the NB/OMC displayed a new well defined redox couple in the potential range of ?250 to 50 mV in pH 6.85 phosphate buffer. Interestingly, we found that only the new redox couple exhibited significant catalytic activity towards the oxidation of NADH. Under a lower operation potential of ?0.1 V, NADH could be linearly detected up to 350 μM with an extremely lower detection limit of 1.2 μM (S/N = 3). |
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