| β-发夹多肽的全新设计和构象研究 |
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| 引用本文： | 沙印林,李银玲,邱阳,王琦,来鲁华,唐有祺.β-发夹多肽的全新设计和构象研究[J].物理化学学报,2002,18(10):907-910. |
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| 作者姓名： | 沙印林 李银玲 邱阳 王琦 来鲁华 唐有祺 |
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| 作者单位： | Single Molecule and Nano-biomedicine Laboratory, Department of Biophysics, School of Basic Medical Sciences, Peking University, Beijing 100083;Institute of Physical Chemistry, Peking University, Beijing 100871 |
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| 基金项目： | 国家攀登计划（970211006）,国家自然科学基金（20103001）资助项目 |
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| 摘 要： | 引入跨股氨基酸队的方法进行β-发夹结构的设计,序列R1G2T3F4W5V6d-P7S8V9N10Y11F12, β2] 中包含二个氨基酸对V6V9和F4Y11,并以d-P7S8作转角来稳定结构.多肽合成采用Fmoc/But固相合成方法.圆二色谱研究显示,β2在202 nm呈现正峰,在217.5 nm处呈负峰,为β转角和β折叠共同贡献的叠加,是典型的β-发夹结构圆二色谱特征.红外光谱研究进一步验证了圆二色谱的结果,表明β2在溶液中主要以β-发夹结构存在.
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| 关 键 词： | 全新设计 β-发夹 β-转角 β-折叠片 跨股氨基酸对 圆二色谱 红外光谱 |
| 收稿时间： | 2002-02-25 |
| 修稿时间： | 2002-04-08 |
de novo Design and
Conformational Studies of a |
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| Abstract.de novo Design and
Conformational Studies of a[J].Acta Physico-Chimica Sinica,2002,18(10):907-910. |
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| Authors: | Abstract |
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| Institution: | Single Molecule and Nano-biomedicine Laboratory, Department of Biophysics, School of Basic Medical Sciences, Peking University, Beijing 100083;Institute of Physical Chemistry, Peking University, Beijing 100871 |
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| Abstract: | A 12-residue peptide (R1G2T3F4W5V6d-P7S8V9N10Y11F12, β2) with cross-strand amino acid pairs V6V9, F4Y11 and Y3F12, predicted to form β-hairpin, was designed and synthesized by Fmoc/But strategy. The circular dichroism spectrum ofβ2 in PBS buffer shows a maximum at about 202 nm and a minimum at about 217.5 nm, a typical β-hairpin characteristics that have been postulated as the common contribution from a β-turn mixed with a β-sheet. The FT-IR experiments confirmed the secondary structural contents of β2. |
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| Keywords: | de novo design β-hairpin' ') β-hairpin" target="_blank">">β-hairpin β-turn' ') β-turn" target="_blank">">β-turn β-sheet' ') β-sheet" target="_blank">">β-sheet Cross-strand amino acid pairs CD FT-IR |
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