| β-转角肽的溶液构象 |
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| 引用本文： | 高峰,沙印林,邱阳,王跃丰,吴厚铭,李银玲,来鲁华.β-转角肽的溶液构象[J].物理化学学报,2001,17(7):619-621. |
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| 作者姓名： | 高峰 沙印林 邱阳 王跃丰 吴厚铭 李银玲 来鲁华 |
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| 作者单位： | Institute of Physical Chemistry,College of Chemistry and Molecular Engineering ,State Key Laboratory for Structural Chemistry of Unstable ＆ Stable Species,Pek ing University,Beijing 100871;Department of Biophysics,The School of Basic Medical Sciences,Peking University,Beijing 100083;Institute of Organic Chemis try,Chinese Academy of Sciences,Shanghai 200032 |
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| 基金项目： | “九五”攀登项目资助(970211006) |
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| 摘 要： | 主要报导TEM-1β-内酰胺酶的天然蛋白类抑制剂BLIP中一段多肽B1的溶液构象研究 结果.在磷酸盐缓冲溶液中，通过圆二色光谱、傅立叶红外光谱和核磁共振谱研究了B1的二 级结构特征.实验结果表明，B1在溶液中形成了β-转角结构，为在溶液中单独研究β-转 角结构形成与稳定性提供了良好的模板.β-转角在溶液中可以独立存在，表明β-转角在 蛋白质折叠过程中可能具有重要作用.
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| 关 键 词： | β-转角结构 圆二色光谱 傅立叶红外光谱 核磁共振光谱 多肽 |
| 收稿时间： | 2001-02-12 |
| 修稿时间： | 2001年2月12日 |
Solution Conformation Study on a |
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| Gao Feng Sha Yin-Lin, Qiu Yang Wang Yue-Feng Li Yin-Ling Lai Lu-Hua Wu Hou-Ming.Solution Conformation Study on a[J].Acta Physico-Chimica Sinica,2001,17(7):619-621. |
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| Authors: | Gao Feng Sha Yin-Lin Qiu Yang Wang Yue-Feng Li Yin-Ling Lai Lu-Hua Wu Hou-Ming |
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| Institution: | Institute of Physical Chemistry,College of Chemistry and Molecular Engineering ,State Key Laboratory for Structural Chemistry of Unstable ＆ Stable Species,Pek ing University,Beijing 100871;Department of Biophysics,The School of Basic Medical Sciences,Peking University,Beijing 100083;Institute of Organic Chemis try,Chinese Academy of Sciences,Shanghai 200032 |
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| Abstract: | The model six-residue linear peptide AAGDYY-NH2 from TEM-1 β-lact amase inhibitor protein and BLIP was predicted to adopt a β-turn conformation and synthesized in order to elucidate the mechanism of β-turn formation and s tability.Its structural preferences in solution were comprehensively characteriz ed using CD (circular dichroism),FT-IR and 1H-NMR spectroscopy.The set of obse rved short- and medium-range NOEs,the restrained molecular dynamics simulation ,CD and FT-IR spectroscopy were consistent with the formation of β-turn in so lution by the model peptide.The results implicate β-turn playing an important role in the process of protein folding. |
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| Keywords: | β-turn' ') β-turn" target="_blank">">β-turn CD FT-IR NMR Peptide |
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