| 固定化辣根过氧化物酶在有机/水混合溶液中的电化学 |
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| 引用本文： | 刘慧宏,万永清,陈显堂,邹国林.固定化辣根过氧化物酶在有机/水混合溶液中的电化学[J].物理化学学报,2006,22(7):868-872. |
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| 作者姓名： | 刘慧宏 万永清 陈显堂 邹国林 |
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| 作者单位： | Department of Chemistry & Biology, Xiangfan University, Xiangfan 441053, P. R. China; College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China |
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| 基金项目： | 中国博士后科学基金;湖北省自然科学基金;湖北省教育厅科研项目 |
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| 摘 要： | 用魔芋多糖(KGM)将辣根过氧化物酶(HRP)固定在玻碳电极(GCE)表面, 制备了HRP-KGM膜修饰电极. 在乙醇等亲水性有机溶剂与水的混合溶液中, 包埋在KGM中的HRP 可以与电极发生直接电子传递, 且能催化还原过氧化氢、氢过氧化异丙基苯、氢过氧化叔丁基、过氧化丁酮等过氧化物. HRP-KGM膜修饰电极具有较好的稳定性和重现性, 可用于这些物质的定量检测.
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| 关 键 词： | 辣根过氧化物酶 电化学 过氧化物 |
| 收稿时间： | 2005-11-16 |
| 修稿时间： | 2006-03-05 |
Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture |
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| LIU Hui-Hong,WAN Yong-Qing,CHEN Xian-Tang,ZOU Guo-Lin.Electrochemistry of Immobilized Horseradish Peroxidase in Organic Solvents/Water Mixture[J].Acta Physico-Chimica Sinica,2006,22(7):868-872. |
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| Authors: | LIU Hui-Hong WAN Yong-Qing CHEN Xian-Tang ZOU Guo-Lin |
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| Institution: | Department of Chemistry & Biology, Xiangfan University, Xiangfan 441053, P. R. China; College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China |
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| Abstract: | Horseradish peroxidase (HRP) was immobilized on glassy carbon electrode (GCE) surface by konjac glucomannan (KGM). HRP entrapped in the KGM film underwent fast direct transfer-electron reactions in water-organic solvent mixtures. The electrocatalysis of peroxides, such as hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide, and 2-butanone peroxide, by the immobilized HRP was also explored. Stability and reproducibility of the modified electrode made it possible to determine the organic peroxides. |
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| Keywords: | Horseradish peroxidase Electrochemistry Peroxide |
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