两种稀土卟啉配合物与大肠杆菌作用的微量热研究

用LKB-2277生物活性检测系统测定了新合成的阳离子型稀土卟啉配合物{Re(TMP)(H2O)3]Cl, Re=Y、Yb, TMP=5, 10, 15, 20-四(4’-甲氧基苯基)卟啉}在37 ℃时对大肠杆菌作用的产热曲线,根据产热曲线求算了在稀土卟啉配合物作用下,大肠杆菌生长代谢的速率常数k,抑制率I,传代时间tG和半抑制浓度cI,50等热动力学参数.结果表明,稀土卟啉配合物在低浓度下对大肠杆菌有刺激作用,高浓度下为抑制作用,Yb(TMP)(H2O)3]Cl的半抑制浓度cI,50为143 mg•L-1,其对大肠杆菌的抑制作用优于Y(TMP)(H2O)3]Cl.

Microcalorimetric Study of the Action of two kinds of Lanthanide Monoporphyrinate Complexes on Growth Metabolism of Escherichia coli

The biothermochemical characteristics of the actions of two kinds of cationic lanthanide(III) monoporphyrinate complexes {Re(TMP)(H2O)3]Cl, Re=Y, Yb, TMP=5,10,15,20 -tetrakis(4’-methoxyphenyl)porphyrin} on growth metabolism of Escherichia coli were studied by means of a LKB-2277 bioactivity monitor at 37 ℃.The maximum power, Pmax; the growth rate constant k1,k2; inhibitive ratio I(％) and half inhibition concentration cI,50 (mg•L-1),respectively, were obtained. Experimental results show that porphyrinate complexes possess the bi-directional biological effection: they stimulate the growth of the E. coli at lower concentration but inhibit the growth at higher concentration. Inhibitory effection of Yb(TMP)(H2O)3]Cl (cI,50=143 mg•L-1) is stronger than that of Y(TMP)(H2O)3]Cl.