| 微量热法测定细菌的临界生长温度 |
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| 引用本文： | 谢昌礼,汤厚宽,宋昭华,屈松生,廖耀庭,刘海水.微量热法测定细菌的临界生长温度[J].物理化学学报,1991(4). |
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| 作者姓名： | 谢昌礼 汤厚宽 宋昭华 屈松生 廖耀庭 刘海水 |
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| 作者单位： | 武汉大学化学系，武汉大学化学系，武汉大学化学系，武汉大学化学系，广州军区武汉总医院，广州军区武汉总医院 武汉 430072，武汉 430072，武汉 430072，武汉 430072 |
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| 摘 要： | 应用微量热学的方法,我们已能成功地测得细菌生长过程的热谱,这种热谱包含着有关细菌生长代谢过程的丰富信息,例如对热谱曲线的指数生长段进行解析,可得出细菌生长的速率常数、激活能和有关的热力学参数。故采用微量热法测定细菌在不同培养温度下的生长速率常数,利用计算机拟合出相应k(速率常数)和T(培养温度)的线性关系式后,若把生长速率为零的温度定义为临界生长温度时,就可以根据由上述实验所得的k～T线性关系式求得细菌的临界生长温度。本工作仍采用微量热法对福氏志贺氏菌(S. flexneri)和大肠埃希氏菌(E. coli)进行实验测定。按文献的方法求出它们在不同温度下的生长速率常数;对于大肠埃希氏菌还用几种不同的培养基分别进行实验测定。
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| 关 键 词： | 微量量热学 生长热潜 临界生长温度 |
CRITICAL GROWTH TEMPERATURES OF BACTERIA BY MICROCALORIMETRIC METHOD |
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| Xie Changli Tang Houkuan Song Zhaohua Qu Songsheng.CRITICAL GROWTH TEMPERATURES OF BACTERIA BY MICROCALORIMETRIC METHOD[J].Acta Physico-Chimica Sinica,1991(4). |
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| Authors: | Xie Changli Tang Houkuan Song Zhaohua Qu Songsheng |
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| Institution: | Xie Changli~* Tang Houkuan Song Zhaohua Qu Songsheng |
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| Abstract: | In this paper, we have determined the multiplication rate constanis (k) of Shigella flexneri and E. coli. at different temperatures and in different culture media by means of a microcalorimeter. From these results a linear equation as k~(1/2)=a+bT can be established for the bacterial growth. The corresponding linear equation for S. flexneri is k~(1/2)=-1.2823+0.0047122 T(r=0.971), so its eritica lgrowth temperature T_0 is equal to 272. 1 K. The linear equation for E coli is k~(1/2)=-1.6390+0.005948 T (r=0.994), T_0=275.5 K. The experimental results indicate that the critial growth temperature of E. coli. is nearly a constant (T_0=273.9K) in different culture media, which is very informative for the study on microorganism growth. |
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| Keywords: | Microcalorimetry Growth thermogram Critial growth temperature |
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