含萘直链多胺化合物的荧光pH效应及核苷磷酸盐的分子识别

合成了三种含萘但链长不同的多胺类化合物.研究了它们在不同pH条件下的荧光光谱，发现所研究化合物的荧光强度与pH值间有着强烈的依赖关系，因此这类化合物可用作测定溶液pH值的荧光探针.在上述化合物溶液中分别引入不同的核苷磷酸盐（ATP、ADP、AMP），发现所研究的化合物如N-(2-氨乙基)-N’-（2-(1-萘甲基)氨乙基]）乙二胺(化合物3)，其荧光可被不同的核苷磷酸盐所猝灭.以Stern-Volmer常数（KSV）或猝灭常数（Kq）作为评估猝灭能力大小的标准表明,不同核苷磷酸盐的猝灭能力是各不相同的.其中ATP有着对化合物3荧光最大的猝灭功能.实验结果表明,分子尺寸的匹配和化合物中基团的空间合理排布是化合物和核苷磷酸盐间能发生相互作用的重要根据,而猝灭则强烈地依赖于这类物种间的相互作用,因此可利用化合物荧光强度的改变来识别各种不同的核苷磷酸盐.

The Ph Effect on the Fluorescence of Linear Poly-amino-compounds and the Recognition of Nucleoside Phosphates

Three kinds of naphthalene containi ng poly-amino-compounds with diffe rent chain length have been synthesized in this work.Their fluorescence spectra were recorded under different pH conditions and sh owed that the fluorescence intensity of s tudied compounds depends on the pH value very strongly.It means that this kind of compounds can be used as a prob e for the determination of pH value of solution.In the solution of these compounds,different kinds of nucle oside phosphate(ATP,ADP and AMP )were introduced respectively.It wa s found that the fluorescence of compounds studied in this work such as C 10 H 7 CH 2 (NHCH 2 CH 2 ) 3 NH 2 (compound3)can be quenched by different nucleoside phosphate and their quenching ab ility which was evaluated by the Stern-Volmer constants or quenchin g constants were also different.The results obtained showed that the ATP has the strongest ability to the fluorescence quenching of compound 3.It dem onstrated that the molecular size matching and the sterically arrangement of groups of studied compounds w ith nucleoside phosphates are very important for the interaction of them.And the quenching ability depends on the interaction of these species strongly.Evidently,this phenomen on can be used as a method for the molecular recognition of nucleoside phosphate by the changing of fluorescence intensity of these compounds.