| 生物热动力学研究——大肠杆菌生长速率和激活能 |
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| 引用本文： | 汤厚宽,谢昌礼,宋昭华,屈松生,廖耀庭,刘海水.生物热动力学研究——大肠杆菌生长速率和激活能[J].物理化学学报,1987,3(2):113-115. |
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| 作者姓名： | 汤厚宽 谢昌礼 宋昭华 屈松生 廖耀庭 刘海水 |
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| 作者单位： | Department of Chemist; Wuhan University;The First Army Hospital of Guangzhou Military Area |
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| 摘 要： | 大肠杆菌生长的全热谱已被测定,再进一步根据其指数生长期的热谱信息,确定了它的生长速率和激活能。在指数生长期,细菌数按指数规律增加,可用下式表示:n_t=n_0e~(kt)(1)式中:t为指数生长期开始后的某一时间,n_0,n_t分别为指数生长期开始时和t时的细菌数,k为生长速率常数。因此,同期细菌输出的热功率也是按指数规律同步增长。可表示为:
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| 收稿时间： | 1986-07-09 |
| 修稿时间： | 1986-09-21 |
STUDIES ON BIOTHERMOKINETICS MEASUEREMENT OF MULTIPLICATION RATE CONSTANTS AND ACTIVATION ENERGY OF ESCHERICHIA COLI USING MICROCALORIMETER |
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| Tang Houkuan,Xie Changli,Song Zhaohua,Qu Songsheng,Liao Yaoting,Liu Haishui.STUDIES ON BIOTHERMOKINETICS MEASUEREMENT OF MULTIPLICATION RATE CONSTANTS AND ACTIVATION ENERGY OF ESCHERICHIA COLI USING MICROCALORIMETER[J].Acta Physico-Chimica Sinica,1987,3(2):113-115. |
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| Authors: | Tang Houkuan Xie Changli Song Zhaohua Qu Songsheng Liao Yaoting Liu Haishui |
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| Institution: | Department of Chemist; Wuhan University;The First Army Hospital of Guangzhou Military Area |
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| Abstract: | In this paper, firstly, we have been studied the multiplication curves of E. coli at exponential phase of growth at 37 ℃ using microcalorimeter, LKB 2277 Bioactivity Monitor, and calculated the multiplication rate constant of E.coli using computer, which is k=0.0397±0.0025 min~(-1). Generation time, G=17.47 minutes, has been obtained from formula G=ln2/k, which is in close agreement with liter- ature value, G=17 minutes. Secondiy, using above method, rate constants of E. coli at different temperatures at exponential of growth have been determined. Finally, according to Arrhenius equation, the activation energy of E. coli multiplication has been obtained, which is 53.09 kJ mol~(-1). |
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