水溶性金属卟啉与DNA相互作用的微量热法研究

叶琳化合物以其对肿瘤组织的特殊亲和性和光动力学效应受到广泛的重视，国内外研究报导甚多·自D79年nel等人山证实水溶性四一K甲基毗陡基）叶咐及其金属配合物能嵌入＊＊A的碱基之间后，人们以这类水溶性叶琳为模型利用各种物理和化学手段研究它们与＊NA相互作用［2，3］．但用微量热法进行研究尚未见报导．我们曾报导用共振拉曼光谱研究图1所示的二个水溶性金属叶琳ru（＊A仰）」和卜i（N＊＊刊I同＊＊A的作用＊‘］，本文进一步报导用微量热法和紫外可见光谱研究的结果．1实验部分1．1试剂将小牛胸腺DN以华美生物工程公司产品）直接…

The Interaction of Water-soluble Quaternary Ammonium Pridine Pophyrins with DNA Studied by Microcalorimetry

The interactions of water-soluble metal porphyrins Cu(NECN) and Ni(NEAE) with calf thymus ds DNA and ss DNA have been investigated by microcalarimetry and UV spectroscopy. The results demonstrat that the reaction of Cu(NACN) with ds DNA is endothermic with the binding enthalpy △H=9.2 kJ?mol-1 for Cu(NACN), its saturated binding number is 4~5 base pairs, the reaction of Ni(NEAE) with ds DNA is exothermal with the binding enthalpy △H=-7.6 kJ?mol-1 for Ni(NEAE), its saturated binding number is 5-6 base pairs, the difference of their binding modes are mainly attributed to their metal ionic properties in porphyrine molecules which are studied in this paper. Watersoluble porphyrins, Cu(NACN) and Ni(NEAE) interact with ss DNA more intensive.