维生素E与磷脂酰胆碱的单分子膜研究

测定了下列气水界面单分子膜的表面压－平均分子面积等温线：（１）ｄ－α，ｄ－β，ｄ－γ－，和ｄ－δ－生育酚等４种维生素Ｅ与ＤＰＰＣ，ＤＯＰＣ及ＤＬＰＣ的混合物，（２）ｄ－α－生育酚等摩尔比的ＤＰＰＣ和ＤＬＰＣ的混合物，（３）胆固醇与ＤＰＰＣ，ＤＬＰＣ的混合物，讨论了维生素Ｅ色满环上甲基对其在ＰＣ单分子膜中物理化作用的影响，实验结果表明：（１）维生素Ｅ以ｄ－α〉ｄ－β－≈ｄ－γ〉ｄ－δ的次序引起的ＤＰ

Study on Monolayer of Vitamin E and Phosphatidylcholines

The surface pressure-area isotherms of mixed monolayers at the air/water interface have been determined for systems: d-α-, d-β-, d-γ-, and d-δ-tocopherol(TOC) with dipalmitoylphosphatidylcholine (DPPC) and dioleoylphosphatidylcholine(DOPC); d-α-TOC and cholesterol (CH) with dilinoleoylphosphatidylcholine (DLPC);and d-α-TOC with DPPC/DLPC (1:1/mol:mol). The effect of the chromanol methyl groups of vitamin E(VE) on its physicochemical behaviors in these monolayers had been discussed. It was observed that (1) TOC caused the DPPC monolayer to expand in the order ofα>β≈γ>δ, and the DOPC monolayer to condense in reverse order; (2) The collapse pressure of monolayer for DOPC and DLPC was not changed by TOC, while that of DPPC decreased; (3) Addition of d-α-TOC into the monolayer of equimolar mixture of DPPC and DLPC did not change the collapse pressure but produced an expanding effect; (4) Contrary to the generally accepted view, the physicochemical function of VE in mixed monolayer of phospholipids was obviously different from that of CH.