Microcalorimetry as a tool for Cr(VI) toxicity evaluation of human dermal fibroblasts |
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Authors: | W Liu F Chaspoul D Berge Lefranc L Decome P Gallice |
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Institution: | (1) Laboratoire PRNT, EA 1784, IFR PMSE 112, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385 Marseille cedex 5, France |
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Abstract: | Occupational exposure to Cr(VI) causes various effects including deep skin ulcerations. Its action mechanisms are not fully
understood. In the present study, the evaluation of human dermal fibroblasts heat production was monitored, using microcalorimetry.
as part of Cr(VI) toxicity. In control cells, normal heat production was 15±5 pW/cell. Regardless of the Cr(VI) concentration
tested (0 to 500 μM), heat production was inhibited over time periods ranging from 3 to 25 h. These results could be correlated
with cell mortality and the IC50 for Cr(VI) was 29±4 μM. In the WST-1 bioassay, the IC50 was 35±5 μM (no statistical difference). Thus, Cr(VI) altered the metabolism of the fibroblasts, and led to cellular death.
Microcalorimetry can be a useful tool for determining the toxic effect of suspect compounds implicated in the occurrence of
pathologies. |
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Keywords: | cytotoxicity assay fibroblasts hexavalent chromium (Cr(VI) microcalorimetry |
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