Application of Coumarin Hydroxylamine in the Fluorescent Quantitative Analysis of Furfural Molecules
Yawei Lin,Junjie Tan,Ying Xu,Wengao Yao,Xiaosong Hu.Application of Coumarin Hydroxylamine in the Fluorescent Quantitative Analysis of Furfural Molecules[J].University Chemistry,2020,35(4):145-151.
Department of Chemistry, School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, P. R. China
Abstract:
Furfural compounds are unique flavor substances in the food industry, but they have a certain carcinogenic effect when accumulated in human body. Therefore, quantitative analysis of furfural compounds is very important in food industry and environmental detection. In this experiment, coumarin hydroxylamine was used as fluorescent label to derive furfural compounds by nitronization reaction, and the contents of furfural, 5-methyl furfural and 5-hydroxymethyl furfural in milk powder were accurately determined by liquid chromatography-fluorescence detection. The method has the advantages of high selectivity, wide linear range and low detection limit. This innovative experiment is based on the latest research results, and is developed into a research design experiment in "comprehensive chemistry laboratory" course of applied chemistry major. Through all-round experimental operation and training, students can fully understand the relationship between fluorescence and molecular structure, the design and selection of fluorescent derivative reagents, the detection principle and application of modern analytical instruments. By optimizing the test conditions and derivative reaction conditions of the instrument, students can learn how to optimize the experimental design and understand the basic process of scientific research. Thus, it can stimulate students' interest in scientific research, improve their operational ability, cultivate their scientific spirit of diligent thinking, and enhance their innovative ability and comprehensive quality.
