Ionic matrix for matrix-enhanced surface-assisted laser desorption ionization mass spectrometry imaging (ME-SALDI-MSI) |
| |
Authors: | Qiang Liu Lin He |
| |
Institution: | 1. Department of Chemistry, North Carolina State University, 27695, Raleigh, NC, USA
|
| |
Abstract: | Matrix-enhanced surface-assisted laser desorption ionization mass spectrometry imaging (ME-SALDI MSI) has been previously
demonstrated as a viable approach to improving MS imaging sensitivity. We describe here the employment of ionic matrices to
replace conventional MALDI matrices as the coating layer with the aims of reducing analyte redistribution during sample preparation
and improving matrix vacuum stability during imaging. In this study, CHCA/ANI (α-cyano-4-hydroxycinnamic acid/aniline) was
deposited atop tissue samples through sublimation to eliminate redistribution of analytes of interest on the tissue surface.
The resulting film was visually homogeneous under an optical microscope. Excellent vacuum stability of the ionic matrix was
quantitatively compared with the conventional matrix. The subsequently improved ionization efficiency of the analytes over
traditional MALDI was demonstrated. The benefits of using the ionic matrix in MS imaging were apparent in the analysis of
garlic tissue sections in the ME-SALDI MSI mode. |
| |
Keywords: | |
本文献已被 ScienceDirect SpringerLink 等数据库收录! |
|