Apparent inhibition by arginine of macrocyclic <Emphasis Type="Italic">b</Emphasis> ion formation from singly charged protonated peptides |
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Authors: | Samuel P Molesworth Michael J Van Stipdonk |
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Institution: | 1.Department of Chemistry,Wichita State University,Wichita,USA |
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Abstract: | There is now strong evidence for the existence of macrocyclic isomers of bn+ ions, the formation and subsequent opening of which can lead to loss of sequence information from protonated peptides in
multiple-stage tandem mass spectrometry experiments. In this study, the fragmentation patterns of protonated YARFLG and permuted
isomers of the model peptide were investigated by collision-induced dissociation. Of interest was the potential influence
of the arginine residue, and its position in the peptide sequence, on formation of the presumed macrocyclic b5 ion isomer and potential loss of sequence information. We find that regardless of the sequence position (either internal
or at the N- or C-terminus), only direct sequence ions or ions directly related to fragmentation of the arginine side chain
are observed. |
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