The mass-mobility correlation redux: The conformational landscape of anhydrous biomolecules |
| |
Authors: | John A McLean |
| |
Institution: | (1) Department of Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt Institute of Integrative Biosystems Research and Education, Vanderbilt University, 7330 Stevenson Center, Nashville, TN 37235, USA; |
| |
Abstract: | Structural separations on the basis of gas-phase ion mobility-mass spectrometry are increasingly used for the analysis of
complex biological samples. As a tool to elucidate biomolecular structure, ion mobility-mass spectrometry methods are unique
in that direct molecular structural information is obtained for all resolved species, largely irrespective of the complexity
of the sample. Computational approaches are used to interpret and discern structural details consistent with the empirical
results. To a first approximation, correlations of mobility with mass allow for qualitative identification of the molecular
class to which a particular species belongs. These correlations allow simultaneous characterization of different classes of
biomolecules, which provides a means for combining omics measurements, such as lipidomics, proteomics, glycomics, and metabolomics,
in the same analysis. Examination of the correlation of fine structure reveals that specific structural motifs, chemical functionality,
chemical connectivity, and composition may also be determined, depending on the specific biomolecular class. Mapping the coarse
and fine structure in ion mobility-mass spectrometry conformation space measurements provides an atlas for interpretation
and discovery in complicated spectra. |
| |
Keywords: | |
本文献已被 ScienceDirect SpringerLink 等数据库收录! |
|